KMID : 0624620150480100559
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BMB Reports 2015 Volume.48 No. 10 p.559 ~ p.564
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Mangiferin inhibits tumor necrosis factor-¥á-induced matrix metalloproteinase-9 expression and cellular invasion by suppressing nuclear factor-¥êB activity
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Dilshara Matharage Gayani
Kang Chang-Hee Choi Yung-Hyun Kim Gi-Young
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Abstract
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We investigated the effects of mangiferin on the expression and activity of metalloproteinase (MMP)-9 and the invasion of tumor necrosis factor (TNF)-¥á-stimulated human LNCaP prostate carcinoma cells. Reverse-transcription polymerase chain reaction (RT-PCR) and western blot analysis showed that mangiferin significantly reversed TNF-¥á-induced mRNA and protein expression of MMP-9 expression. Zymography data confirmed that stimulation of cells with TNF-¥á significantly increased MMP-9 activity. However, mangiferin substantially reduced the TNF-¥á-induced activity of MMP-9. Additionally, a matrigel invasion assay showed that mangiferin significantly reduced TNF-¥á-induced invasion of LNCaP cells. Compared to untreated controls, TNF-¥á-stimulated LNCaP cells showed a significant increase in nuclear factor-¥êB (NF-¥êB) luciferase activity. However, mangiferin treatment markedly decreased TNF-¥á-induced NF-¥êB luciferase activity. Furthermore, mangiferin suppressed nuclear translocation of the NF-¥êB subunits p65 and p50. Collectively, our results indicate that mangiferin is a potential anti-invasive agent that acts by suppressing NF-¥êB-mediated MMP-9 expression.
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KEYWORD
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Invasion, Mangiferin, Matrix metalloproteinase-9, Nuclear factor kappa B
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